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Literature summary extracted from

  • Fiorini, C.; Gotte, G.; Donnarumma, F.; Picone, D.; Donadelli, M.
    Bovine seminal ribonuclease triggers Beclin1-mediated autophagic cell death in pancreatic cancer cells (2014), Biochim. Biophys. Acta, 1843, 976-984.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 G38K the mutant is more basic and interacts more strongly with the acidic membrane of cancer cells compared to the wild-type enzyme Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.18 RNase inhibitor RNase A, like most monomeric RNases, is strongly bound and inactivated in mammalian cells by the RNase inhibitor Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-
4.6.1.18 semen
-
 Bos taurus
-

Subunits

EC Number Subunits Comment Organism
4.6.1.18 dimer constituted by two identical subunits covalently bound through two antiparallel disulfide bridges Bos taurus
4.6.1.18 monomer
-
 Bos taurus
4.6.1.18 More native RNase A can be induced to form various N- or C-terminal domain-swapped dimers, trimers, and larger oligomers, all reconstituting the active site and augmenting the enzymatic activity against dsRNA with respect to the native monomer. RNase A oligomers can also acquire cytotoxic activity both in vitro and in vivo Bos taurus
4.6.1.18 oligomer the enzyme can oligomerize through the 3D domain swapping of both N- and C-termini, and its multimers is enzymatically and biologically more active than the native dimer Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 bovine seminal RNase
-
 Bos taurus
4.6.1.18 BS-RNase
-
 Bos taurus
4.6.1.18 RNase A
-
 Bos taurus
4.6.1.18 Seminal RNase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.18 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.18 7.4
-
 assay at Bos taurus

General Information

EC Number General Information Comment Organism
4.6.1.18 evolution the enzyme belongs to the pancreatic-type secretory ribonuclease superfamily Bos taurus
4.6.1.18 evolution the enzyme belongs to the pancreatic-type secretory ribonuclease superfamily as a unique natively dimeric member Bos taurus
4.6.1.18 physiological function the enzyme has almost no anti-tumoral property in pancreatic adenocarcinoma cell lines and in nontumorigenic cells as normal control, and is largely ineffective as anti-proliferative and pro-apoptotic agent Bos taurus
4.6.1.18 physiological function the enzyme has good anti-tumoral property in pancreatic adenocarcinoma cell lines and in nontumorigenic cells as normal control, it stimulates a strong anti-proliferative and pro-apoptotic effect in cancer cells. The enzyme triggers Beclin1-mediated autophagic cancer cell death, providing evidence that high proliferation rate of cancer cells may render them more susceptible to autophagy by treatment with the enzyme Bos taurus